|
KMID : 0379120120400040191
|
|
Korean Journal of Mycology
2012 Volume.40 No. 4 p.191 ~ p.203
|
|
|
Effects of Medium, Temperature and pH on Mycelial Growth and Cellulase Activity of Ectomycorrhizal Fungi from Korean Forests
|
|
Jeon Sung-Min
Kim Min-Soo
Ka Kang-Hyeon
|
|
|
Abstract
|
|
|
|
Mycelial growth of ectomycorrhizal fungi (27 strains of 8 species) collected from Korean forests was observed on various culture conditions (media, temperature, pHs). After 60 days of incubation, all strains grown on potato dextrose agar (PDA) and modified Melin-Norkran’s agar (MMNA), whereas no mycelial growth was observed on malt extract agar (MEA) or sabouraud dextrose agar (SDA) in some strains including Tricholoma matsutake. Mycelial growth on PDA was poor at high temperature (30o C) than the low temperature (10o C). The optimal temperature on PDA and pH in potato dextrose broth (PDB) for mycelial growth in most strains were 20-25o C and pH 4-5, respectively. All strains tested showed the carboxymethyl cellulase (CM-cellulase) activity and the maximal cellullase activity was expressed by the mycelium of T. matsutake (KFRI 1266) on the CMC agar plate with pH 5.0.
|
|
|
KEYWORD
|
|
|
Cardinal temperature, Cellulase, Ectomycorrhizal fungi, Mycelial growth, pH
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|